In dna, a purine must always pair with a pyrimidine and vice versa in order to ensure that5/16/2023 Under our conditions, the TFOs bind to their cognate double strand DNAs with binding constants of 2.6 × 10 5 and 2.3 × 10 6 M -1. We have analyzed triplex formation of two polypurine triplex forming oligodeoxynucleotides (TFOs) using fluorescence resonance energy transfer (FRET). Sequence specific triplex formation can be applied for gene targeting, gene silencing and mutagenesis. A third DNA strand can bind into the major groove of a homopurine duplex DNA to form a DNA triple helix.
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